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VE-Cadherin Antibody (BV13) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43348

Novus Biologicals, part of Bio-Techne
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NBP1-43348-0.05mg
NBP1-43348-0.1mg
NBP1-43348-0.5mg
Conjugate
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # BV13

Format

BSA Free

Concentration

0.5 mg/ml

Product Specifications

Immunogen

The immunogen for this antibody was CD144.

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for VE-Cadherin Antibody (BV13) - BSA Free

Flow Cytometry: VE-Cadherin Antibody (BV13) - BSA Free [NBP1-43348]

Flow Cytometry: VE-Cadherin Antibody (BV13) - BSA Free [NBP1-43348]

Flow Cytometry: VE-Cadherin Antibody (BV13) [NBP1-43348] - Analysis using the Biotin conjugate of NBP1-43348. Staining of bEnd.3 cell line with 0.5 micrograms of Rat IgG1 k Isotype Control Biotin (blue histogram) or 0.125 micrograms of Anti-Mouse CD144 (VE-Cadherin) Biotin purple histogram) followed by Streptavidin PE. Total viable cells were used for analysis.
Flow Cytometry: VE-Cadherin Antibody (BV13) - BSA Free [NBP1-43348]

Flow Cytometry: VE-Cadherin Antibody (BV13) - BSA Free [NBP1-43348]

Flow Cytometry: VE-Cadherin Antibody (BV13) [NBP1-43348] - Staining of bEnd.3 cell line with 0.5 ug of Rat IgG1 K Isotype Control Purified (open histogram) or 0.5 ug of AntiMouse CD144 (VE-Cadherin) Purified (filled histogram) followed by F(ab')2 Anti-Rat IgG PE. Total viable cells were used for analysis.

Applications for VE-Cadherin Antibody (BV13) - BSA Free

Application
Recommended Usage

Flow Cytometry

< = 1 ug/10^6 cells in 100 ul

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Intravenous injection of BV13 has been shown to induce a concentration- and timedependent increase in vascular permeability in the heart and lungs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VE-Cadherin

The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Entrez Gene IDs

1003 (Human)

Gene Symbol

CDH5

UniProt

Additional VE-Cadherin Products

Product Documents for VE-Cadherin Antibody (BV13) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VE-Cadherin Antibody (BV13) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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