VEGF-C Antibody

Western Blot: VEGF-C Antibody [NB110-61022] -

Western Blot: VEGF-C Antibody [NB110-61022] - Kallikrein-related peptidase 3 (KLK3)/Prostate specific antigen (PSA) activates VEGF-C.(A, B) Cleavage of pro-VEGF-C by KLK3 (PSA). Pro-VEGF-C was incubated with or without KLK3, with & without the monoclonal antibody against KLK3 (5C7). Detection of VEGF-C in Western blots probed with antiserum 6 & 3/4, resulting in the detection of pro-VEGF-C (29/31 kDa) & activated, mature VEGF-C (21/23 kDa). The band marked by the asterisk likely represents the N-terminal propeptide (~15 kDa) which is detected by the antiserum 6. Note that for the image shown for antiserum 6, two different exposures of the same blot were merged (n = 3). (C, D) VEGF-C processed by KLK3 is biologically active in Ba/F3 cell assays, which translate activation of a hybrid VEGFR/EpoR receptor into cell survival (n = 2). Error bars indicate SD.10.7554/eLife.44478.004Figure 1—source data 1.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C.Ba/F3 assay showing the activity of KLK3-cleaved VEGF-C. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31099754), licensed under a CC-BY license. Not internally tested by Novus Biologicals.