VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43978

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL0345

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all VEGFR1/Flt-1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P17948, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free

VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NBP3-43978]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NBP3-43978]

Analysis in human placenta and tonsil tissues using NBP3-43978 antibody. Corresponding VEGFR1/Flt-1 RNA-seq data are presented for the same tissues.
VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free Western Blot: VEGFR1/Flt-1 Antibody [NBP3-43978]

Western Blot: VEGFR1/Flt-1 Antibody [NBP3-43978]

Lane 1: Marker [kDa]
Lane 2: Human cell line RT-4
VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NBP3-43978]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NBP3-43978]

Staining of human kidney shows strong cytoplasmic positivity in endothelial cells.
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Applications for VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR1/Flt-1

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VEGFR1/Flt-1 Antibody (CL0345) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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