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VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21963

Novus Biologicals, part of Bio-Techne
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NBP2-21963-0.025mg

Key Product Details

Species Reactivity

Human, Mouse

Applications

Block/Neutralize, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MAB0702

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a mouse immunized with purified extracellular domain of recombinant human VEGFR1. VEGFR-1 ( Antagonistic) EC domain, AAC50060.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

This antibody recognizes both human and mouse VEGFR1 in western blot. It shows no cross-reactivity with human or mouse VEGFR2.

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free

Western Blot: VEGFR1/Flt-1 Antibody (MAB0702)Azide and BSA Free [NBP2-21963]

Western Blot: VEGFR1/Flt-1 Antibody (MAB0702)Azide and BSA Free [NBP2-21963]

Western Blot: VEGF R1/Flt-1 Antibody (MAB0702) [NBP2-21963] - HUVECs, preincubated with anti-VEGFR1 at indicated dose for 20mins, were stimulated with human VEGF for 20 mins and then the level of p-VEGFR1 was detected using anti-phospho-VEGFR1 (Y1213).

Applications for VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:400-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR1/Flt-1

Receptors for VEGF, VEGFB and PGF have tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. VEGF and its high-affinity binding receptors--the tyrosine kinases FLK1 and FLT1--are thought to be important for the development of embryonic vasculature. It has been shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

UniProt

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VEGFR1/Flt-1 Antibody (MAB0702) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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