VEGFR1/Flt-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-527

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Predicted:

Chicken (94%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all VEGFR1/Flt-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the mouse VEGF Receptor 1 protein (between residues 800-900) . There is 87% identity between the immunogen used for this production and the VEGF Receptor 2 protein.

Localization

Type 1 membrane protein (isoform Flt1)

Specificity

This antibody targets VEGFR-1 but has significant cross-reactivity with the VEGFR-2 protein.

Marker

Endothelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VEGFR1/Flt-1 Antibody

Western Blot: VEGFR1/Flt-1 Antibody [NB100-527]

Western Blot: VEGFR1/Flt-1 Antibody [NB100-527]

Western Blot: VEGF R1/Flt-1 Antibody [NB100-527] - Chimeric CSF-1R/VEGFR-2 detection in transfected lysates.
Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527]

Immunocytochemistry/ Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527]

Immunocytochemistry/Immunofluorescence: VEGFR1/Flt-1 Antibody [NB100-527] - VEGF R1/Flt-1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527]

Immunohistochemistry-Paraffin: VEGFR1/Flt-1 Antibody [NB100-527] - Analysis of FFPE human breast carcinoma tissue section using 1:500 dilution of VEGFR1/Flt-1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). This VEGFR1/Flt-1 antibody generated an expected membrane cytoplasmic staining of VEGFR1 protein in the cancer cells (punctate appearance typical of receptors). The tumor stroma/stromal cells did not show VEGFR1/Flt-1immunopositivity.
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Applications for VEGFR1/Flt-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:250 - 1:500

Immunohistochemistry-Paraffin

1:250 - 1:500

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: VEGFR1/Flt-1

VEGF Receptor 1 (vascular endothelial growth factor receptor 1 or VEGFR1; also called Flt1 in mouse) is a tyrosine-protein kinase which acts as a cell-surface receptor for VEGFA, VEGFB and PGF ligands for playing an essential role in the embryonic vasculature development and regulation of angiogenesis (normal as well as cancerous tissues), cell survival, migration, macrophage function, chemotaxis, and tumor invasion. VEGFR1 is a 180-185 kD glycoprotein expressed primarily in vascular endothelial cells and also found in a wide range of non-endothelial cells, such as monocytes and macrophages, human trophoblasts, renal mesangial cells, vascular smooth muscle cells, dendritic cells and different human tumour cell types. Interestingly, VEGFR1 acts as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells, whereas it promotes endothelial cell proliferation, survival and angiogenesis in adults. VEGFR1 expression is regulated by hypoxia through HREs in VEGFR1 promoter. It exists in an inactive conformation in the absence of bound ligand and binding of VEGFA, VEGFB or PGF leads to its dimerization followed by tyrosine residue autophosphorylation mediated activation. VEGFR1 phosphorylation sites includes Tyr794, Tyr1169, Tyr1213, Tyr1242, Tyr1327, Tyr1333 etc. and VEGFR1 phosphorylation pattern depends on the ligand, e.g. PlGF, but not VEGFA, induces phosphorylation of Tyr1309. VEGFR1 has been shown to exert its diverse biological activities through its effects on multiple signal transduction pathways including VEGF, KDR, PLCG, PI3K, MAPK etc.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Alternate Names

Flt-1, FLT1, FRT, VEGF R1, VEGFR-1

Gene Symbol

FLT1

Additional VEGFR1/Flt-1 Products

Product Documents for VEGFR1/Flt-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VEGFR1/Flt-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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