VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18646
![Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-20I6-Immunocytochemistry-Immunofluorescence-NBP1-18646-img0015.jpg "Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]")
![Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6)Azide and BSA Free [NBP1-18646]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-20I6-Western-Blot-NBP1-18646-img0006.jpg "Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6)Azide and BSA Free [NBP1-18646]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 20I6
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Summary for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free
Immunogen
Recombinant human soluble extracellular KDR (D7)
Specificity
The monoclonal antibody will detect native human VEGFR-2/KDR in ELISA experiments and on the surface of different human cell types.
Marker
Endothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Clone #4 changed to 20I6 for a more unique identifier.
Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free
Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6)Azide and BSA Free [NBP1-18646]
Western Blot: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Recombinant human endogenous soluble VEGFR-2/KDR (esKDR) was produced in insect cells. Western blot was performed using our monoclonal anti-VEGFR-2 recognizing the soluble as well as the transmembrane form of KDR (left panal) and our new poyclonal antibody directed against the unique C-terminal end of the endogenous sKDR (CGRETILDHSAEAVGMP) recognizing solely the endogenous form but not sKDR (D7) and sKDR (D7)-Fc consisting of the full extraplasmatic domain. The endogenous sKDR generated by alternative splicing consists of the first 6 Ig-like loops with a unique c-terminal end.![Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-20I6-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-18646-img0016.jpg "Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]")
Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]
Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - HMEC-1 CRL-3243 cells, stained with 1:50 VEGFR2/KDR/Flk-1 antibody (NBP1-18645AF488) (green) and DAPI (blue). Image from verified customer review.![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-20I6-Flow-Cytometry-NBP1-18646-img0009.jpg "Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]")
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646]
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free [NBP1-18646] - VEGFR2/KDR/Flk-1 Antibody (20I6) [NBP1-18646] - Analysis with primary human dermal lymphatic endothelial cells(HDLEC).Applications for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-10 ug/ml
Flow Cytometry
2-5 ug/ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
1:10-1:500
Western Blot
2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VEGFR2/KDR/Flk-1
Long Name
Vascular Endothelial Growth Factor Receptor 2
Alternate Names
CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2
Entrez Gene IDs
3791 (Human)
Gene Symbol
KDR
Additional VEGFR2/KDR/Flk-1 Products
Product Documents for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free
Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (20I6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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