VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36429

Novus Biologicals, part of Bio-Techne
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NBP2-36429-0.025mg
NBP2-36429-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2C6

Format

BSA Free

Concentration

1.0 mg/ml

View all VEGFR2/KDR/Flk-1 Antibodies »

Product Specifications

Immunogen

Partial recombinant human VEGFR2 protein (between amino acids 300-600) [UniProt# P35968].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free

Western Blot: VEGFR2/KDR/Flk-1 Antibody (2C6)BSA Free [NBP2-36429]

Western Blot: VEGFR2/KDR/Flk-1 Antibody (2C6)BSA Free [NBP2-36429]

Western Blot: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of VEGF R2/KDR/Flk-1 (clone 2C6) in partial recombinant protein.
Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free [NBP2-36429]

Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free [NBP2-36429]

Immunocytochemistry/Immunofluorescence: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Antibody (1:50) was tested in HUVEC cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free [NBP2-36429]

Immunohistochemistry-Paraffin: VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free [NBP2-36429]

Immunohistochemistry-Paraffin: VEGF R2/KDR/Flk-1 Antibody (2C6) [NBP2-36429] - Analysis of FFPE tissue section of a malignant stromal tumor of the human small bowel with antibody at 5 ug/ml concentration. The cancer cells depicted a specific and strong staining in the cytoplasm.
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Applications for VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VEGFR2/KDR/Flk-1

VEGF receptor 2 is a member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. Like other growth factor receptors, upon ligand binding VEGF receptor 2 dimerises and is autophosphorylated on multiple tyrosine residues. These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2 and phosphotyrosine binding containing signalling proteins. Phosphorylation of Tyrosines 1054 and 1059 in the activation loop is required for activation of VEGF receptor 2 and its intrinsic tyrosine kinase activity. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

Long Name

Vascular Endothelial Growth Factor Receptor 2

Alternate Names

CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2

Entrez Gene IDs

3791 (Human)

Gene Symbol

KDR

UniProt

P35968

Additional VEGFR2/KDR/Flk-1 Products

Product Documents for VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (2C6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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