VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-18644
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0006.jpg "ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
![ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-ELISA-NBP1-18644-img0018.jpg "ELISA: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
![Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-Immunocytochemistry-Immunofluorescence-NBP1-18644-img0017.jpg "Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 4H3
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant human soluble extracellular KDR (D7)
Specificity
The monoclonal antibody will detect native human VEGFR-2/KDR on the surface of different human cell types.
Marker
Endothelial Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Clone #3 changed to 4H3.
Scientific Data Images for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]
Immunocytochemistry/Immunofluorescence: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644] - VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - IF double staining of human KDR in a co-culture of PAE-Flt-1, PAE-KDR and PAE-FLT-4 with (A) mouse anti-human KDR, (B) rabbit antihuman KDR (C) DAPI and (D) merged. Conjugated secondary antibody: goat anti-rabbit ALEXA Flour (1:600) and goat anti-mouse PE.![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0011.jpg "Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Analysis using the Biotin conjugate of NBP1-18644. Staining of VEGFR-2/KDR expression in HUVE cells.![Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]](https://resources.bio-techne.com/images/products/VEGFR2-KDR-Flk-1-Antibody-4H3-Flow-Cytometry-NBP1-18644-img0008.jpg "Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]")
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free [NBP1-18644]
Flow Cytometry: VEGFR2/KDR/Flk-1 Antibody (4H3) [NBP1-18644] - Fig. 1: Flow analysis of VEGF R2/KDR/Flk-1 expression in primary human dermal mivrovascular endothelial cells (HDMVEC) and human umbilical vein endothelial cells (HUVEC).Applications for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5 ug/ml
Flow Cytometry
2 - 5 ug/ml
Immunocytochemistry/ Immunofluorescence
1-5 ug/ml
Western Blot
1-2 ug/ml
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VEGFR2/KDR/Flk-1
Long Name
Vascular Endothelial Growth Factor Receptor 2
Alternate Names
CD309, Flk-1, Flk1, KDR, KRD1, Ly73, VEGF R2
Entrez Gene IDs
3791 (Human)
Gene Symbol
KDR
UniProt
Additional VEGFR2/KDR/Flk-1 Products
Product Documents for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
Product Specific Notices for VEGFR2/KDR/Flk-1 Antibody (4H3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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