VG5Q Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-454

Novus Biologicals, part of Bio-Techne
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NB100-454
NB100-454SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all VG5Q Antibodies »

Product Specifications

Immunogen

A synthetic peptide mapping to an epitope within residues 500-600 of the human VG5Q protein. [UniProt# Q8N302]

Localization

Cytoplasm

Specificity

This is specific for human VG5Q.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VG5Q Antibody

Western Blot: VG5Q Antibody [NB100-454]

Western Blot: VG5Q Antibody [NB100-454]

Western Blot: VG5Q Antibody [NB100-454] - Human VG5Q detected in HT1080 non-transfected cell lysate (NTC) and HT1080 transfected cell lysate (TC) using NB 100-454.
Immunohistochemistry-Paraffin: VG5Q Antibody [NB100-454]

Immunohistochemistry-Paraffin: VG5Q Antibody [NB100-454]

Immunohistochemistry-Paraffin: VG5Q Antibody [NB100-454] - VG5Q was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human VG5Q polyclonal Antibody (Catalog # NB100-454) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in hepatocytes.

Applications for VG5Q Antibody

Application
Recommended Usage

ELISA

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

reported in scientific literature

Immunohistochemistry

1:250

Immunoprecipitation

reported in scientific literature

Western Blot

1:500
Application Notes
This VG5Q antibody can be used for Immunohistochemistry and Western Blot, where a specific band is seen at 100 kDa. Other bands are non-specific or a degradation product (~75 kDa). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VG5Q

VG5Q (vasculogenesis gene on 5q protein, also called AGGF1) is a novel angiogenic factor that has been identified through genetic analysis of Klippel-Trenaunay syndrome (KTS), a congenital vascular disorder composed of capillary malformations, venous malformations or varicose veins, and hypertrophy of the affected tissues. VG5Q is broadly expressed in different cell types including endothelial cells (umbilical vein endothelial cells and microvascular endothelial cells), vascular smooth muscle cells and osteoblasts. VG5Q expression is mainly cytoplasmic and upon angiogenic stimulation especially when endothelial cell tube formation is initiated, VG5Q gets secreted and interact with a secreted angiogenic factor TNFSF12. VG5Q promotes angiogenesis as well as the proliferation of endothelial cells through direct binding in an autocrine fashion. Moreover, it has been demonstrated to promote angiogenesis as potentially as VEGF and its expression decreases with knockdown of GATA1 gene which is proposed as VG5Q regulator through two GATA1 binding sites in VG5Q promoter region.

Long Name

Vasculogenesis Gene on 5q/Angiogenic Factor with G Patch and FHA Domains 1

Alternate Names

AGGF1

Entrez Gene IDs

55109 (Human)

Gene Symbol

AGGF1

UniProt

Q8N302

Additional VG5Q Products

Product Documents for VG5Q Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VG5Q Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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