VGLUT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1837

Novus Biologicals, part of Bio-Techne
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NB100-1837-0.05mg
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Canine

Cited:

Rat, Canine

Applications

Validated:

Electron Microscopy, ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Radioimmunoassay, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all VGLUT1/SLC17A7 Antibodies »

Product Specifications

Immunogen

A 17 residue synthetic peptide derived from C-terminal domain of rat VGLUT1.

Reactivity Notes

Not yet tested in other species. Use in Canine reported in scientific literature (PMID:18558516).

Localization

Cell Membrane

Specificity

Reacts with rat 55-60 kDa VGLUT1 protein of BON cells stably expressing VGLUT1 and of total rat brain extracts. No cross reactivity was detected with VGLUT2 and VGLUT3

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VGLUT1 Antibody

Western Blot: VGLUT1 Antibody [NB100-1837]

Western Blot: VGLUT1 Antibody [NB100-1837]

Western Blot: VGLUT1 Antibody [NB100-1837] - Cell lysate from rat

Applications for VGLUT1 Antibody

Application
Recommended Usage

ELISA

1:100-1:2000

Electron Microscopy

1:200

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Radioimmunoassay

1:500

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

Lyophilized powder, reconstituted in distilled water.

Formulation

Tris 0,1M, glycine 0,1M, sucrose 2%.

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGLUT1/SLC17A7

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Entrez Gene IDs

116638 (Rat)

Gene Symbol

SLC17A7

UniProt

Q62634 (Rat)

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VGLUT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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