VGLUT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33597

Novus Biologicals, part of Bio-Techne
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NBP2-33597
NBP2-33597-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VGLUT1/SLC17A7 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MEFRQEEFRKLAGRALGKLHRL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VGLUT1 Antibody

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Analysis in human cerebral cortex and pancreas tissues. Corresponding VGLUT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human cerebellum, cerebral cortex, pancreas and testis using Anti-VGLUT1 antibody NBP2-33597 (A) shows similar protein distribution across tissues to independent antibody NBP2-39000 (B).
Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597]

Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-33597] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
View 3 more images

Applications for VGLUT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VGLUT1/SLC17A7

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate.

Long Name

Vesicular Glutamate Transporter 1

Alternate Names

BNPI, SLC17A7

Gene Symbol

SLC17A7

UniProt

Q9P2U7

Additional VGLUT1/SLC17A7 Products

Product Documents for VGLUT1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VGLUT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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