Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # S29-29

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VGLUT2 Antibodies »

Product Summary for VGLUT2 Antibody (S29-29)

Immunogen

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2

Localization

Cytoplasmic Vesicle , Secretory Vesicle , Synaptic Vesicle Membrane , Membrane , Cell Junction , Synapse

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VGLUT2 Antibody (S29-29)

Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330]

Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330]

Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:000.
Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330]

Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330]

Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT2 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330]

Immunocytochemistry/ Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330]

Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.
View 1 more images

Applications for VGLUT2 Antibody (S29-29)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: VGLUT2

VGLUT2 is a sodium dependent inorganic phosphate cotransporter that is involved in the calcium dependent glutamate release from astrocytes. It is expressed in only a subset of neurons, localized to synaptic vesicles, at synapses exhibiting classical excitatory features. VGLUT2 mRNA is found in brain regions that lack VGLUT1, a related protein also displaying sodium dependent inorganic phosphate cotransporter activity.

Alternate Names

differentiation-associated BNPI, differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, differentiation-associated Na-dependent inorganic phosphate cotransporter, DNPI, solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6, solute carrier family 17 member 6, vesicular glutamate transporter 2, VGLUT2

Gene Symbol

SLC17A6

Additional VGLUT2 Products

Product Documents for VGLUT2 Antibody (S29-29)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VGLUT2 Antibody (S29-29)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.