VIAAT/SLC32A1/VGAT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-740

Novus Biologicals, part of Bio-Techne
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NB300-740
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat, Chicken, Xenopus

Predicted:

Bovine (100%), Canine (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VIAAT/SLC32A1 Antibodies »

Product Summary for VIAAT/SLC32A1/VGAT Antibody

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # O35458

Reactivity Notes

It is anticipated that the antibody will also work with bovine, canine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~53 kDa VIAAT/SLC32A1/VGAT protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VIAAT/SLC32A1/VGAT Antibody

Western Blot: VIAAT/SLC32A1/VGAT Antibody [NB300-740]

Western Blot: VIAAT/SLC32A1/VGAT Antibody [NB300-740]

Western Blot: VIAAT/SLC32A1/VGAT Antibody [NB300-740] - Analysis of VIAAT/SLC32A1/VGAT in rat hippocampal lysate.

Applications for VIAAT/SLC32A1/VGAT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:100 - 1:1000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VIAAT/SLC32A1

The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Acid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

Long Name

Vesicular Inhibitory Amino Acid Transporter

Alternate Names

SLC32A1, VGAT

Entrez Gene IDs

50676 (Rat)

Gene Symbol

SLC32A1

UniProt

O35458 (Rat)

Additional VIAAT/SLC32A1 Products

Product Documents for VIAAT/SLC32A1/VGAT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VIAAT/SLC32A1/VGAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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