Villin 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32841

Novus Biologicals, part of Bio-Techne
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NBP1-32841
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Feline

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Villin 1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Villin 1. The exact sequence is proprietary.

Reactivity Notes

Cat (100%).

Localization

Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Villin 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Villin 1 Antibody [NBP1-32841]

Immunocytochemistry/ Immunofluorescence: Villin 1 Antibody [NBP1-32841]

Immunocytochemistry/Immunofluorescence: Villin 1 Antibody [NBP1-32841] - Villin 1 and Cadherin-17 were detected in immersion fixed, paraffin-embedded sections of MimEX adult stem cell-derived ascending colon tissue with Mouse Anti-Human Cadherin-17 Monoclonal Antibody (Catalog # MAB1032) at 1.25 ug/mL and Rabbit Anti-Human/Mouse/Rat/Bovine Villin 1 Antibody (Catalog # NBP1-32841) at 0.62 ug/mL overnight at 4 C. Tissue was then stained using the NorthernLights 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and NorthernLights 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Cadherin-17 staining was detected in cellular membranes throughout the epithelial layer while Villin 1 staining was localized specifically to the apical surface of the polarized epithelial layer.
Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841]

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841]

Immunohistochemistry-Paraffin: Villin 1 Antibody [NBP1-32841] - Rat colon. Villin stained by Villin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: Villin 1 Antibody [NBP1-32841]

Western Blot: Villin 1 Antibody [NBP1-32841]

Western Blot: Villin 1 Antibody [NBP1-32841] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGE Villin antibody dilution: 1:2000
View 13 more images

Applications for Villin 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Villin 1

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon.

Alternate Names

D2S1471, VIL, villin 1, villin-1

Gene Symbol

VIL1

Additional Villin 1 Products

Product Documents for Villin 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Villin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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