Vinculin Antibody (VCL/2575)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-79808
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # VCL/2575
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary) (Uniprot: P18206)
Localization
Cytoplasmic
Marker
Marker of Age-related Macular Degeneration
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79942)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Vinculin Antibody (VCL/2575)
Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808]
Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human U-87, THP-1 cell lysate using Vinculin Antibody (VCL/2575).Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2575) [NBP2-79808]
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/2575) [NBP2-79808] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Vinculin Antibody (VCL/2575).Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808]
Western Blot: Vinculin Antibody (VCL/2575) [NBP2-79808] - Western Blot Analysis of human A431, K562, NIH3T3 cell lysate using Vinculin Antibody (VCL/2575).Applications for Vinculin Antibody (VCL/2575)
Application
Recommended Usage
Immunohistochemistry
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Vinculin
Additional Vinculin Products
Product Documents for Vinculin Antibody (VCL/2575)
Product Specific Notices for Vinculin Antibody (VCL/2575)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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