Vinculin Antibody (VCL/7091R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13989
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989]](https://resources.bio-techne.com/images/products/Vinculin-Antibody-VCL-7091R-Immunohistochemistry-Paraffin-NBP3-13989-img0001.jpg "Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # VCL/7091R
Concentration
0.2 mg/ml
Product Summary for Vinculin Antibody (VCL/7091R)
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary) (Uniprot: P18206)
Localization
Cytoplasm
Marker
Marker of Age-related Macular Degeneration
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14197)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Vinculin Antibody (VCL/7091R)
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989]
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989] - Formalin-fixed, paraffin-embedded human placentastained with Vinculin antibody (VCL/7091R) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.![Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989]](https://resources.bio-techne.com/images/products/Vinculin-Antibody-VCL-7091R-Immunohistochemistry-Paraffin-NBP3-13989-img0002.jpg "Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989]")
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989]
Immunohistochemistry-Paraffin: Vinculin Antibody (VCL/7091R) [NBP3-13989] - Formalin-fixed, paraffin-embedded human pancreasstained with Vinculin antibody (VCL/7091R) at 2ug/ml.Applications for Vinculin Antibody (VCL/7091R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Vinculin
Additional Vinculin Products
Product Documents for Vinculin Antibody (VCL/7091R)
Product Specific Notices for Vinculin Antibody (VCL/7091R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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