Vinculin Antibody (VCL/7091R) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14197
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # VCL/7091R
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Vinculin Antibody (VCL/7091R) - Azide and BSA Free
Immunogen
Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein (exact sequence is proprietary) (Uniprot: P18206)
Localization
Cytoplasm
Marker
Marker of Age-related Macular Degeneration
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
117 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13989).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Vinculin Antibody (VCL/7091R) - Azide and BSA Free
Vinculin Antibody (VCL/7091R)
Formalin-fixed, paraffin-embedded human placenta stained with Vinculin antibody (VCL/7091R) at 2ug/ml. Inset: PBS instead of primary antibody; secondary only negative control.
Vinculin Antibody (VCL/7091R)
Formalin-fixed, paraffin-embedded human pancreas stained with Vinculin antibody (VCL/7091R) at 2ug/ml.Applications for Vinculin Antibody (VCL/7091R) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Vinculin
Long Name
Metavinculin
Alternate Names
MVCL, VCL
Gene Symbol
VCL
Additional Vinculin Products
Product Documents for Vinculin Antibody (VCL/7091R) - Azide and BSA Free
Product Specific Notices for Vinculin Antibody (VCL/7091R) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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