Vinculin Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41237

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Vinculin Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Vinculin. The immunogen is located within amino acids 1060-1110 of Vinculin. Amino Acid Sequence: LGRTNISDEESEQATE

Specificity

Vinculinantibody is human, mouse and rat specific. At least three isoforms of Vinculinare known to exist. Human Vinculinhas 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculinhas one isoform (1066aa, 117kD) and Rat Vinculinalso has one isoform (1066aa, 117kD). NBP2-41237 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

124 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Vinculin Antibody - BSA Free

Western Blot: Vinculin AntibodyBSA Free [NBP2-41237]

Western Blot: Vinculin AntibodyBSA Free [NBP2-41237]

Western Blot: Vinculin Antibody [NBP2-41237] - Western Blot Validation in Mouse 3T3/balb Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry: Vinculin Antibody - BSA Free [NBP2-41237]

Immunohistochemistry: Vinculin Antibody - BSA Free [NBP2-41237]

Immunohistochemistry: Vinculin Antibody - BSA Free [NBP2-41237] - Vinculin Antibody [NBP2-41237] - Rat small intestine tissue with Vinculin antibody at 20 ug/mL.
Western Blot: Vinculin AntibodyBSA Free [NBP2-41237]

Western Blot: Vinculin AntibodyBSA Free [NBP2-41237]

Western Blot: Vinculin Antibody [NBP2-41237] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (2 ug/mL), Vinculin from Company B (1 ug/mL), Vinculin NBP2-41274 (1 ug/mL), and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Applications for Vinculin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/mL

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Vinculin

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. (provided by RefSeq)

Long Name

Metavinculin

Alternate Names

MVCL, VCL

Gene Symbol

VCL

Additional Vinculin Products

Product Documents for Vinculin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Vinculin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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