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Key Product Details

Species Reactivity

Validated:

Human, Rat, Porcine, Bovine

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

Vasoactive intestinal peptide

Specificity

Binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for VIP Antibody (2) - BSA Free

Immunocytochemistry/ Immunofluorescence: VIP Antibody (2) - BSA Free [NBP1-05163]

Immunocytochemistry/ Immunofluorescence: VIP Antibody (2) - BSA Free [NBP1-05163]

Immunocytochemistry/Immunofluorescence: VIP Antibody (2) [NBP1-05163] - Staining in human pancreas tissue. Signal is strong in nerves of exocrine part of pancreas. Dilution: 1:100.
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]

Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]

Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163] - Formalin fixed paraffin-embedded human pancreas tissue . Dilution: 1:100
Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]

Immunohistochemistry-Paraffin: VIP Antibody (2) - BSA Free [NBP1-05163]

Immunohistochemistry-Paraffin: VIP Antibody (2) [NBP1-05163] - Staining of human appendix tissue. Dilution: 1:100

Applications for VIP Antibody (2) - BSA Free

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:100-1:2000
Application Notes
ELISA: A dilution guideline of 1/1000 applies to VIP coated directly at 2 ug/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2 ug/mL, the dilution is 1/32,000. WB: NBP1-05163 can be used in Western blotting. IHC: NBP1-05163 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:100. IF: NBP1-05163 was used in IF with a dilution of 1:100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: VIP

Vasoactive intestinal peptide (VIP) is a 28-amino-acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Long Name

Vasoactive Intestinal Peptide

Alternate Names

MGC13587

Gene Symbol

VIP

Additional VIP Products

Product Documents for VIP Antibody (2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VIP Antibody (2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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