Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

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Product Specifications

Immunogen

Full length human recombinant protein of human VIP (NP_003372) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

16.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VIP Antibody (OTI1E8) - Azide and BSA Free

Western Blot: VIP Antibody (OTI1E8)Azide and BSA Free [NBP2-74848]

Western Blot: VIP Antibody (OTI1E8)Azide and BSA Free [NBP2-74848]

Western Blot: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VIP.
Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848]

Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848]

Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848]

Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848]

Immunohistochemistry: VIP Antibody (OTI1E8) - Azide and BSA Free [NBP2-74848] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
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Applications for VIP Antibody (OTI1E8) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VIP

Vasoactive intestinal peptide (VIP) is a 28-amino-acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Long Name

Vasoactive Intestinal Peptide

Alternate Names

MGC13587

Gene Symbol

VIP

Additional VIP Products

Product Documents for VIP Antibody (OTI1E8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VIP Antibody (OTI1E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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