VIP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12900

Novus Biologicals, part of Bio-Techne
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NBP3-12900
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Porcine (100%), Rat (100%), Rhesus Macaque (100%), Sheep (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VIP Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human VIP. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for VIP Antibody

Western Blot: VIP Antibody [NBP3-12900]

Western Blot: VIP Antibody [NBP3-12900]

Western Blot: VIP Antibody [NBP3-12900] - VIP antibody detects VIP protein by western blot analysis. Whole cell extracts (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP antibody (NBP3-12900) at a dilution of 1:1000.
Immunohistochemistry-Frozen: VIP Antibody [NBP3-12900]

Immunohistochemistry-Frozen: VIP Antibody [NBP3-12900]

Immunohistochemistry-Frozen: VIP Antibody [NBP3-12900] - VIP antibody detects VIP protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebral cortex. Green: VIP stained by VIP antibody (NBP3-12900) diluted at 1:250. Blue: Fluoroshield with DAPI.

Applications for VIP Antibody

Application
Recommended Usage

Immunohistochemistry-Frozen

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VIP

Vasoactive intestinal peptide (VIP) is a 28-amino-acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in man, ox, pig and rat. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome. VIP shows high sequence homology with peptide histidine-methionine (PHM) in man or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Long Name

Vasoactive Intestinal Peptide

Alternate Names

MGC13587

Gene Symbol

VIP

Additional VIP Products

Product Documents for VIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for VIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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