Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Virilizer Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1760 and 1810 of human Virilizer (KIAA1429) using the numbering given in entry NP_056311.2 (GeneID 25962).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Virilizer Antibody - BSA Free

Western Blot: Virilizer Antibody [NBP1-21364]

Western Blot: Virilizer Antibody [NBP1-21364]

Western Blot: Virilizer Antibody [NBP1-21364] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Virilizer antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Virilizer Antibody [NBP1-21364]

Immunoprecipitation: Virilizer Antibody [NBP1-21364]

Immunoprecipitation: Virilizer Antibody [NBP1-21364] - Detection of human Virilizer by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Virilizer antibody NBP1-21364 used for IP at 6 ug per reaction. Virilizer was also immunoprecipitated by another rabbit anti-Virilizer antibody. For blotting immunoprecipitated Virilizer, NBP1-21364 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Virilizer Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Virilizer

The virilizer (vir) gene was discovered as a factor required for dosage compensation and sex determination in female flies. In drosophila it has been determined to function as a positive regulator of female-specific mRNA splicing. Database searches using the deduced amino acid sequence of the drosophila VIR retrieved a human protein BAA92667 (GenBank) which corresponds to the gene designated as KIAA1429. The human protein is 28% identical and about 40% similar to drosophila VIR and contains the putative transmembrane domain of drosophila VIR.

Alternate Names

DKFZp434I116, DKFZp781B2117, fSAP121, functional spliceosome-associated protein 121, KIAA1429, MGC138493, MGC141940, protein virilizer homolog

Entrez Gene IDs

25962 (Human)

Gene Symbol

VIRMA

UniProt

NP_056311.2 (Human)

Additional Virilizer Products

Product Documents for Virilizer Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Virilizer Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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