visinin-like 1 Antibody (3A9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25167

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A9

Concentration

1 mg/ml

View all VSNL1 Antibodies »

Product Specifications

Immunogen

Full length human visinin-like 1 [UniProt# P62760]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for visinin-like 1 Antibody (3A9)

Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167]

Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167]

Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167] - Analysis of different tissue lysates using mouse mAb to visinin-like protein 1 (VLP1), NBP2-25167, dilution 1:1,000, in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] pig hippocampus, and [5] cow cerebellum. The band at about 20kDa corresponds to the VLP1 protein.
Immunocytochemistry/ Immunofluorescence: visinin-like 1 Antibody (3A9) [NBP2-25167]

Immunocytochemistry/ Immunofluorescence: visinin-like 1 Antibody (3A9) [NBP2-25167]

Immunocytochemistry/Immunofluorescence: visinin-like 1 Antibody (3A9) [NBP2-25167] - Confocal image of adult rat cerebellum stained with NBP2-25167 (green), chicken polyclonal antibody to MAP2 NB300-213 (red) and DNA (blue). NBP2-25167 reveals perikarya and synaptic regions in the neuron rich granular layer (bottom) and synapse rich molecular layer (top). Note that the large prominent Purkinje neurons at the junction of these two layers do not stain with this antibody, in line with the finidngs of others.
Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167]

Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167]

Western Blot: visinin-like 1 Antibody (3A9) [NBP2-25167] - Bovine cerebellum homogenate stained with NBP2-25167 in lane 11. Note the strong clean band running at 22 kDa. Lane 10 shows the same material stained with another antibody to visinin-like 1, NBP2-25166, which binds to the same band.

Applications for visinin-like 1 Antibody (3A9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500-1:1000

Immunohistochemistry

1:500-1:1000

Western Blot

1:1000-1:2000
Application Notes
This visinin-like 1 (3A9) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western Blot, where a band can be seen at ~22 kDa.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VSNL1

Visinin was originally isolated biochemically from chicken retina as a major protein of about 24 kDa on SDS-PAGE. Following cloning and sequencing of visinin, several visinin-like proteins were discovered by homology screening. One of these, visinin-like 1 is a small calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression. It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites. The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1. The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like 1 has four "EF hand" domains, which are negatively charged helix-turn-helix peptides that are responsible for calcium binding. Visinin-like 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of ~22kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer's disease and traumatic brain injury.

Long Name

Visinin Like 1

Alternate Names

HPCAL3, HUVISL1, VILIP, VISL1

Entrez Gene IDs

7447 (Human)

Gene Symbol

VSNL1

OMIM

600817 (Human)

UniProt

P62760 (Human)

Additional VSNL1 Products

Product Documents for visinin-like 1 Antibody (3A9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for visinin-like 1 Antibody (3A9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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