Vitronectin Antibody (ST49-02)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67511
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # ST49-02
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human Vitronectin aa 429-478 / 478. (SwissProt: P04004 Human; SwissProt: P29788 Mouse; SwissProt: Q3KR94 Rat)
Localization
Secreted.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Vitronectin Antibody (ST49-02)
Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511]
Western Blot: Vitronectin Antibody (ST49-02) [NBP2-67511] - Analysis of Vitronectin on different lysates using anti-Vitronectin antibody at 1/1,000 dilution. Positive control: Lane 1: Human liver Lane 2: Human serumImmunocytochemistry/ Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511]
Immunocytochemistry/Immunofluorescence: Vitronectin Antibody (ST49-02) [NBP2-67511] - Staining Vitronectin in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511]
Immunohistochemistry-Paraffin: Vitronectin Antibody (ST49-02) [NBP2-67511] - Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Vitronectin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-39, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.Applications for Vitronectin Antibody (ST49-02)
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunohistochemistry-Paraffin
1:50-1:400
Western Blot
1:1000-1:5000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
TBS (pH7.4), 0.05% BSA, 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Vitronectin
Alternate Names
Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN
Gene Symbol
VTN
Additional Vitronectin Products
Product Documents for Vitronectin Antibody (ST49-02)
Product Specific Notices for Vitronectin Antibody (ST49-02)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...