Vitronectin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-20866
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Vitronectin. The exact sequence is proprietary.
Localization
Secreted; extracellular space
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Vitronectin Antibody
![Western Blot: Vitronectin Antibody [NBP2-20866]](https://resources.bio-techne.com/images/products/Vitronectin-Antibody-Western-Blot-NBP2-20866-img0009.jpg "Western Blot: Vitronectin Antibody [NBP2-20866]")
Western Blot: Vitronectin Antibody [NBP2-20866]
Western Blot: Vitronectin Antibody [NBP2-20866] - Proteomics analysis of losartan-mediated reversion of early fibrotic RDEB changes.Bar graph show abundance of representative proteins normalized by losartan treatment. Mean +/- S.E.M. (LFQ intensity) of groups of individual mice (wild-type, untreated, & losartan-treated C7-hypomorphic mice). Unpaired t-test calculated significance. Abundance of Lrg1: *P-value C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.022; abundance of serpin f2: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0084; abundance of vitronectin: **P-value untreated C7-hypomorphic vs. losartan-treated C7-hypomorphic mice = 0.0094. Western blots of skin lysates from wild-type, untreated C7-hypomorphic, & 7-week losartan-treated C7-hypomorphic mice not used for proteomics. Image collected & cropped by CiteAb from the following publication (www.onlinelibrary.wiley.com/doi/10.15252/emmm.201505061) licensed under a CC-BY license.![Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866]](https://resources.bio-techne.com/images/products/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0008.jpg "Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866]")
Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866]
Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Vitronectin antibody detects Vitronectin protein by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Vitronectin stained by Vitronectin antibody diluted at 1:1000.![Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866]](https://resources.bio-techne.com/images/products/Vitronectin-Antibody-Immunohistochemistry-Paraffin-NBP2-20866-img0003.jpg "Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866]")
Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866]
Immunohistochemistry-Paraffin: Vitronectin Antibody [NBP2-20866] - Human colon carcinoma, using Vitronectin antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.Applications for Vitronectin Antibody
Application
Recommended Usage
ELISA
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Vitronectin
Alternate Names
Complement S-protein, Serum Spreading Factor, Somatomedin B, VTN
Gene Symbol
VTN
UniProt
Additional Vitronectin Products
Product Specific Notices for Vitronectin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.