Von Hippel Lindau Antibody (1G12)

Novus Biologicals, part of Bio-Techne | Catalog # H00007428-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1G12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VHL Antibodies »

Product Specifications

Immunogen

VHL (NP_000542, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIH

Specificity

VHL - von Hippel-Lindau tumor suppressor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Von Hippel Lindau Antibody (1G12)

Western Blot: Von Hippel Lindau Antibody (1G12) [H00007428-M01]

Western Blot: Von Hippel Lindau Antibody (1G12) [H00007428-M01]

Western Blot: Von Hippel Lindau Antibody (1G12) [H00007428-M01] - Analysis of VHL expression in transfected 293T cell line by VHL monoclonal antibody (M01), clone 1G12.Lane 1: VHL transfected lysate(19.7 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Von Hippel Lindau Antibody (1G12) [H00007428-M01]

Immunocytochemistry/ Immunofluorescence: Von Hippel Lindau Antibody (1G12) [H00007428-M01]

Immunocytochemistry/Immunofluorescence: Von Hippel Lindau Antibody (1G12) [H00007428-M01] - Analysis of monoclonal antibody to VHL on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Von Hippel Lindau Antibody (1G12) [H00007428-M01] - Detection limit for recombinant GST tagged VHL is approximately 0.1ng/ml as a capture antibody.

Applications for Von Hippel Lindau Antibody (1G12)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VHL

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Long Name

Von Hippel-Lindau Tumor Suppressor

Alternate Names

Protein G7, pVHL

Entrez Gene IDs

7428 (Human)

Gene Symbol

VHL

OMIM

171300 (Human)

UniProt

NP_000542

Additional VHL Products

Product Documents for Von Hippel Lindau Antibody (1G12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Von Hippel Lindau Antibody (1G12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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