VPS11 Antibody (8L3D8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15923
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 8L3D8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human VPS11 (Q9H270). MAAYLQWRRFVFFDKELVKEPLSNDGAAPGATPASGSAASKFLCLPPGITVCDSGRGSLVFGDMEGQIWFLPRSLQLTGFQAYKLRVTHLYQLKQHNILA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for VPS11 Antibody (8L3D8)
Western Blot: VPS11 Antibody (8L3D8) [NBP3-15923]
Western Blot: VPS11 Antibody (8L3D8) [NBP3-15923] - Western blot analysis of extracts of various cell lines, using VPS11 Rabbit mAb (NBP3-15923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.Immunohistochemistry-Paraffin: VPS11 Antibody (8L3D8) [NBP3-15923]
Immunohistochemistry-Paraffin: VPS11 Antibody (8L3D8) [NBP3-15923] - Immunohistochemistry of paraffin-embedded mouse stomach using VPS11 Rabbit mAb (NBP3-15923) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Western Blot: VPS11 Antibody (8L3D8) [NBP3-15923]
Western Blot: VPS11 Antibody (8L3D8) [NBP3-15923] - Western blot analysis of extracts of various cell lines, using VPS11 Rabbit mAb (NBP3-15923) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.Applications for VPS11 Antibody (8L3D8)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: VPS11
Alternate Names
hVPS11, PEP5, RING finger protein 108, RNF108END1, vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting 11 homolog (S. cerevisiae), vacuolar protein sorting protein 11, vacuolar protein sorting-associated protein 11 homolog
Gene Symbol
VPS11
Additional VPS11 Products
Product Documents for VPS11 Antibody (8L3D8)
Product Specific Notices for VPS11 Antibody (8L3D8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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