VPS11 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2412
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-HALQEKDTQTKLE corresponding to internal region according to NP_068375.3.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for VPS11 Antibody
![Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]](https://resources.bio-techne.com/images/products/VPS11-Antibody-Immunohistochemistry-Paraffin-NB100-2412-img0002.jpg "Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]")
Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]
Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - (4ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.![Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]](https://resources.bio-techne.com/images/products/VPS11-Antibody-Immunohistochemistry-Paraffin-NB100-2412-img0001.jpg "Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]")
Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412]
Immunohistochemistry-Paraffin: VPS11 Antibody [NB100-2412] - 3.8ug/ml staining of paraffin embedded Human Kidney-. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for VPS11 Antibody
Application
Recommended Usage
Immunohistochemistry
3 - 6 ug/ml
Immunohistochemistry-Paraffin
3 - 6 ug/ml
Peptide ELISA
Detection limit 1:32000
Western Blot
1:100 - 1:2000
Application Notes
WB: Preliminary experiments gave an approx. 75 kDa band in human duodenum, colon and placenta lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 108 kDa band according to NP_068375.3. The 75 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human kidney shows brushborder staning in PCT cells and human pancreas shows cytoplasm staining of beta cells in islets of langerhans.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: VPS11
Alternate Names
hVPS11, PEP5, RING finger protein 108, RNF108END1, vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting 11 homolog (S. cerevisiae), vacuolar protein sorting protein 11, vacuolar protein sorting-associated protein 11 homolog
Gene Symbol
VPS11
UniProt
Additional VPS11 Products
Product Documents for VPS11 Antibody
Product Specific Notices for VPS11 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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