VPS11 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31054

Novus Biologicals, part of Bio-Techne
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NBP1-31054
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Fish (100%), Mouse (100%), Rat (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS11 Antibodies »

Product Summary for VPS11 Antibody

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human VPS11. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (85%), Japanese Medaka (100%), Xenopus Tropicalis (92%).

Localization

Late endosome membrane, Peripheral membrane protein, Cytoplasmic side, Lysosome membrane, Peripheral membrane protein, Cytoplasmic side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS11 Antibody

Western Blot: VPS11 Antibody [NBP1-31054]

Western Blot: VPS11 Antibody [NBP1-31054]

Western Blot: VPS11 Antibody [NBP1-31054] - Sample(30 ug of whole cell lysate)A:293TB:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: VPS11 Antibody [NBP1-31054]

Immunocytochemistry/ Immunofluorescence: VPS11 Antibody [NBP1-31054]

Immunocytochemistry/Immunofluorescence: VPS11 Antibody [NBP1-31054] - Sample: HeLa cells were fixed in -20C 100% MeOH for 5 min. Green: VPS11 protein stained by VPS11 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: VPS11 Antibody [NBP1-31054]

Immunohistochemistry-Paraffin: VPS11 Antibody [NBP1-31054]

Immunohistochemistry-Paraffin: VPS11 Antibody [NBP1-31054] - VPS11 antibody detects VPS11 protein at cytosol on Ca922 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Ca922 xenograft. VPS11 antibody (NBP1-31054).Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for VPS11 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS11

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps11 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway.

Alternate Names

hVPS11, PEP5, RING finger protein 108, RNF108END1, vacuolar protein sorting 11 (yeast homolog), vacuolar protein sorting 11 homolog (S. cerevisiae), vacuolar protein sorting protein 11, vacuolar protein sorting-associated protein 11 homolog

Gene Symbol

VPS11

Additional VPS11 Products

Product Documents for VPS11 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VPS11 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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