VPS34 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86862

Novus Biologicals, part of Bio-Techne
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NBP1-86862
NBP1-86862-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VPS34/PIK3C3 Antibodies »

Product Summary for VPS34 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDIKNGLEPTKKDSQSSVSENVSNSGINSAEIDSSQIITSPLPSVSSPPPASKTKEVPDGENLEQDLCTFLISRACKNSTLANYLYWYVIVECEDQDTQQRDPKTHEMYLNVMRRFSQALLKGDK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS34 Antibody

Western Blot: VPS34 Antibody [NBP1-86862]

Western Blot: VPS34 Antibody [NBP1-86862]

Western Blot: VPS34 Antibody [NBP1-86862] - Analysis in control (vector only transfected HEK293T lysate) and PIK3C3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862]

Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862]

Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862]

Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862]

Immunohistochemistry-Paraffin: VPS34 Antibody [NBP1-86862] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
View 1 more images

Applications for VPS34 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS34/PIK3C3

VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Alternate Names

PI 3-Kinase C3, PI3K type 3, PIK3C3

Gene Symbol

PIK3C3

Additional VPS34/PIK3C3 Products

Product Documents for VPS34 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS34 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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