VPS34 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94870

Novus Biologicals, part of Bio-Techne
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NBP2-94870-0.02ml
NBP2-94870-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all VPS34/PIK3C3 Antibodies »

Product Summary for VPS34 Antibody - BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human VPS34 (NP_002638.2). EADGSEPTKTPGRTSSTLSEDQMSRLAKLTKAHRQGHMVKVDWLDRLTFREIEMINESEKRSSNFMYLMVEFRCVKCDDKEYGIVYYEKDGDESSPILTSF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

101 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS34 Antibody - BSA Free

Western Blot: VPS34 AntibodyBSA Free [NBP2-94870]

Western Blot: VPS34 AntibodyBSA Free [NBP2-94870]

Western Blot: VPS34 Antibody [NBP2-94870] - Analysis of extracts of various cell lines, using VPS34 at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunocytochemistry/ Immunofluorescence: VPS34 Antibody - BSA Free [NBP2-94870]

Immunocytochemistry/ Immunofluorescence: VPS34 Antibody - BSA Free [NBP2-94870]

Immunocytochemistry/Immunofluorescence: VPS34 Antibody [NBP2-94870] - Immunofluorescence analysis of PC-12 cells using VPS34 antibody (NBP2-94870) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for VPS34 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:200-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS34/PIK3C3

VPS34, also known as phosphoinositide-3-kinase p100 subunit or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. VPS34 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. It exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. The activity of VPS34 is enhanced by manganese and can be regulated by nutrients, suggesting an important role of VPS34 in the regulation of mTOR protein synthesis. VPS34 also forms a complex with Beclin1 that promotes autophagy and tumor supression.

Alternate Names

PI 3-Kinase C3, PI3K type 3, PIK3C3

Gene Symbol

PIK3C3

Additional VPS34/PIK3C3 Products

Product Documents for VPS34 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VPS34 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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