VPS41 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2425

Novus Biologicals, part of Bio-Techne
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NB100-2425
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all VPS41 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DHLKKDSQNKTLLK corresponding to internal region according to NP_055211.2, NP_542198.2.

Specificity

This antibody is expected to recognise both reported isoforms (NP_055211.2 and NP_542198.2)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

99 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for VPS41 Antibody

Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425]

Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425]

Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Prostate. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425]

Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425]

Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Kidney. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining. Similar results were obtained after antigen retrieval at pH9.
Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425]

Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425]

Immunohistochemistry-Paraffin: VPS41 Antibody [NB100-2425] - Staining of paraffin embedded Human Placenta. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for VPS41 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

3.75 - 6 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

1 ug/mL
Application Notes
WB: Preliminary experiments gave bands at approx. 48 kDa band and 28 kDa band in human duodenum, kidney and placenta lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 98.5 kDa band according to NP_055211.1 and 100 kDa band according to NP_542198.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). IHC-P: Human kidney shows staining of collective tubules.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: VPS41

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human ortholog of yeast Vps41 protein which is also conserved in Drosophila, tomato, and Arabidopsis. Expression studies in yeast and human indicate that this protein may be involved in the formation and fusion of transport vesicles from the Golgi. Several transcript variants encoding different isoforms have been described for this gene, however, the full-length nature of not all is known. (provided by RefSeq)

Alternate Names

HVPS41, hVps41p, HVSP41, S53, vacuolar assembly protein 41, vacuolar protein sorting 41 (yeast homolog), vacuolar protein sorting 41 (yeast), vacuolar protein sorting 41 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 41 homolog

Entrez Gene IDs

27072 (Human)

Gene Symbol

VPS41

UniProt

P49754 (Human)

Additional VPS41 Products

Product Documents for VPS41 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for VPS41 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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