Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Chicken (94%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all VPS53 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human VPS53. The immunogen is located within the last 50 amino acids of VPS53. Amino Acid Squence: LTAPTPEQESSRIRKLEK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VPS53 Antibody - BSA Free

Western Blot: VPS53 AntibodyBSA Free [NBP1-76901]

Western Blot: VPS53 AntibodyBSA Free [NBP1-76901]

Western Blot: VPS53 Antibody [NBP1-76901] - 293 cell lysate with VPS53 antibody at (A) 0.5 and (B) 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: VPS53 Antibody - BSA Free [NBP1-76901]

Immunocytochemistry/ Immunofluorescence: VPS53 Antibody - BSA Free [NBP1-76901]

Immunocytochemistry/Immunofluorescence: VPS53 Antibody [NBP1-76901] - 293 cells with VPS53 antibody at 5 ug/ml.
Immunocytochemistry/ Immunofluorescence: VPS53 Antibody - BSA Free [NBP1-76901]

Immunocytochemistry/ Immunofluorescence: VPS53 Antibody - BSA Free [NBP1-76901]

Immunocytochemistry/Immunofluorescence: VPS53 Antibody [NBP1-76901] - 293 cells.
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Applications for VPS53 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

2.5-5 ug/ml

Western Blot

0.5-1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VPS53

The sorting of acid hydrolases to lysosomes rely on mannose 6-phosphate receptors that cycle between the trans-Golgi network (TGN) and endosomes. The maintenance of this cycle requires the function of the mammalian Golgi-associated retrograde protein (GARP) complex which is composed of three subunits: VPS52, VPS53, and VPS54. Depletion of any of these three proteins, such as by RNAi, impairs the retrograde transport of multiple TGN proteins. VPS53 was identified as an HIV dependency factor (HDF) and plays a role in viral entry to the cell, suggesting that VPS53 may be an important drug target in HIV treatment. At least five isoforms of VPS53 are known to exist.

Alternate Names

FLJ10979, FLJ41112, FLJ61757, HCCS1, hVps53L, MGC39512, pp13624, vacuolar protein sorting 53 (yeast), vacuolar protein sorting 53 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 53 homolog

Gene Symbol

VPS53

UniProt

EAW90658 (Human)

Additional VPS53 Products

Product Documents for VPS53 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VPS53 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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