VPS53 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97767
![Western Blot: VPS53 Antibody [NBP2-97767]](https://resources.bio-techne.com/images/products/VPS53-Antibody-Western-Blot-NBP2-97767-img0002.jpg "Western Blot: VPS53 Antibody [NBP2-97767]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human VPS53 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for VPS53 Antibody
Western Blot: VPS53 Antibody [NBP2-97767]
Western Blot: VPS53 Antibody [NBP2-97767] - Anti-VPS53 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 95 kDa![Immunoprecipitation: VPS53 Antibody [NBP2-97767]](https://resources.bio-techne.com/images/products/VPS53-Antibody-Immunoprecipitation-NBP2-97767-img0001.jpg "Immunoprecipitation: VPS53 Antibody [NBP2-97767]")
Immunoprecipitation: VPS53 Antibody [NBP2-97767]
Immunoprecipitation: VPS53 Antibody [NBP2-97767] - VPS53 was immunoprecipitated using: Lane A: 0.5 mg U-251 MG Whole Cell Lysate 4 ul anti-VPS53 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-VPS53 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 80 kDa. Observed band size: 95 kDaApplications for VPS53 Antibody
Application
Recommended Usage
Immunoprecipitation
0.5-2uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: VPS53
Alternate Names
FLJ10979, FLJ41112, FLJ61757, HCCS1, hVps53L, MGC39512, pp13624, vacuolar protein sorting 53 (yeast), vacuolar protein sorting 53 homolog (S. cerevisiae), vacuolar protein sorting-associated protein 53 homolog
Gene Symbol
VPS53
Additional VPS53 Products
Product Specific Notices for VPS53 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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