VRK2 Antibody (3B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00007444-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VRK2 Antibodies »

Product Specifications

Immunogen

VRK2 (AAH27854, 260 a.a. ~ 360 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VQTAKTNLLDELPQSVLKWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINVHTPNSQKVDSQKAATKQVNKAHNRLI

Specificity

VRK2 - vaccinia related kinase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for VRK2 Antibody (3B10)

Western Blot: VRK2 Antibody (3B10) [H00007444-M01]

Western Blot: VRK2 Antibody (3B10) [H00007444-M01]

Western Blot: VRK2 Antibody (3B10) [H00007444-M01] - VRK2 monoclonal antibody (M01), clone 3B10 Analysis of VRK2 expression in K-562.
Immunocytochemistry/ Immunofluorescence: VRK2 Antibody (3B10) [H00007444-M01]

Immunocytochemistry/ Immunofluorescence: VRK2 Antibody (3B10) [H00007444-M01]

Immunocytochemistry/Immunofluorescence: VRK2 Antibody (3B10) [H00007444-M01] - Analysis of monoclonal antibody to VRK2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: VRK2 Antibody (3B10) [H00007444-M01]

Western Blot: VRK2 Antibody (3B10) [H00007444-M01]

Western Blot: VRK2 Antibody (3B10) [H00007444-M01] - Analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi ( Cat # H00007444-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody (M01) clone 3B10 (Cat # H00007444-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Applications for VRK2 Antibody (3B10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: VRK2

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. While several transcript variants may exist for this gene, the full-length nature of only one has been biologically validated to date.

Alternate Names

EC 2.7.11.1, serine/threonine-protein kinase VRK2, vaccinia related kinase 2, vaccinia virus B1R-related kinase 2, Vaccinia-related kinase 2, vaccinia-related kinase-2

Entrez Gene IDs

7444 (Human)

Gene Symbol

VRK2

OMIM

602169 (Human)

UniProt

AAH27854 (Human)

Additional VRK2 Products

Product Documents for VRK2 Antibody (3B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for VRK2 Antibody (3B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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