VSIG1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81074

Novus Biologicals, part of Bio-Techne
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NBP1-81074-25ul
NBP1-81074
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all VSIG1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QAVAIGQFKDRITGSNDPGNASITISHMQPADSGIYICDVNNPPDFLGQNQGILNVSVLVKPSKPLCSVQGRPETGHTISLSCLSALGTPSPVYYWHKLEGRDIVPV

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for VSIG1 Antibody

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074] - Staining in human stomach and liver tissues using anti-VSIG1 antibody. Corresponding VSIG1 RNA-seq data are presented for the same tissues.
Western Blot: VSIG1 Antibody [NBP1-81074]

Western Blot: VSIG1 Antibody [NBP1-81074]

Western Blot: VSIG1 Antibody [NBP1-81074] - Analysis in control (vector only transfected HEK293T lysate) and vSIG1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074]

Immunohistochemistry-Paraffin: VSIG1 Antibody [NBP1-81074] - Staining of human liver shows low expression as expected.
View 2 more images

Applications for VSIG1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. VSIG1antibody validated for WB from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NBP1-81074 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: VSIG1

The VSIG1 gene encodes a member of the junctional adhesion molecule (JAM) family. The encoded protein contains multipleglycosylation sites at the N-terminal region, and multiple phosphorylation sites and glutamic acid/proline (EP)repeats at the C-terminal regi

Long Name

V-Set and Immunoglobulin Domain Containing 1

Alternate Names

GPA34

Entrez Gene IDs

340547 (Human)

Gene Symbol

VSIG1

UniProt

Q86XK7

Additional VSIG1 Products

Product Documents for VSIG1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for VSIG1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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