Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all WAPL Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 1125 and 1175 of human wings apart like/KIAA0261 using the numbering given in TrEMBL entry Q7Z5K2 (GeneID 23063).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WAPL Antibody - BSA Free

Western Blot: WAPL Antibody [NB100-552]

Western Blot: WAPL Antibody [NB100-552]

Western Blot: WAPL Antibody [NB100-552] - Detection of human WAPL by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-WAPL antibody NB100-552 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for WAPL Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2500-1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. Western Blot: 1:2,500 - 1:10,000 Immunoprecipitation: not tested Histo/Cytochemistry: not tested *In some cases, the antibody may be diluted further than indicated.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WAPL

The hWAPL mRNA was predominantly expressed in uterine cervical cancer, with weak expression in all other normal and tumor tissues examined. hWAPL expression in benign epithelia was confined to the basal cell layers, whereas in dysplasias it increasingly appeared in more superficial cell layers and showed a significant correlation with severity of dysplasia. Diffuse hWAPL expression was found in all invasive squamous cell carcinomas examined. These results demonstrate that hWAPL is associated with cell growth, and the hWAPL expression may play a significant role in cervical carcinogenesis and tumor progression.

Alternate Names

FOEKIAA0261friend of EBNA2 (Epstein-Barr virus nuclear protein 2), Friend of EBNA2 protein, WAPLwings apart-like protein homolog, wings apart-like homolog (Drosophila)

Entrez Gene IDs

23063 (Human)

Gene Symbol

WAPL

UniProt

Q7Z5K2 (Human)

Additional WAPL Products

Product Documents for WAPL Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WAPL Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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