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WASF2 Antibody (7A6C10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15806

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15806-100ul
NBP3-15806-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7A6C10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 399-498 of human WASF2 (Q9Y6W5). PPGPPPPPFTGADGQPAIPPPLSDTTKPKSSLPAVSDARSDLLSAIRQGFQLRRVEEQREQEKRDVVGNDVATILSRRIAVEYSDSEDDSSEFDEDDWSD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WASF2 Antibody (7A6C10)

Western Blot: WASF2 Antibody (7A6C10) [NBP3-15806]

Western Blot: WASF2 Antibody (7A6C10) [NBP3-15806]

Western Blot: WASF2 Antibody (7A6C10) [NBP3-15806] - Western blot analysis of extracts of various cell lines, using WAVE2/WASF2 Rabbit mAb (NBP3-15806) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: WASF2 Antibody (7A6C10) [NBP3-15806]

Immunohistochemistry-Paraffin: WASF2 Antibody (7A6C10) [NBP3-15806]

Immunohistochemistry-Paraffin: WASF2 Antibody (7A6C10) [NBP3-15806] - Immunohistochemistry of paraffin-embedded mouse testis using WAVE2/WASF2 Rabbit mAb (NBP3-15806) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: WASF2 Antibody (7A6C10) [NBP3-15806]

Immunohistochemistry-Paraffin: WASF2 Antibody (7A6C10) [NBP3-15806]

Immunohistochemistry-Paraffin: WASF2 Antibody (7A6C10) [NBP3-15806] - Immunohistochemistry of paraffin-embedded rat kidney using WAVE2/WASF2 Rabbit mAb (NBP3-15806) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for WASF2 Antibody (7A6C10)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WASF2

WASF2 encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X.

Alternate Names

IMD2, Protein WAVE-2, SCAR2suppressor of cyclic-AMP receptor (WASP-family), Verprolin homology domain-containing protein 2, WAS protein family, member 2, WASP family Verprolin-homologous protein 2, WAVE2dJ393P12.2, wiskWASP family protein member 2

Gene Symbol

WASF2

Additional WASF2 Products

Product Documents for WASF2 Antibody (7A6C10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WASF2 Antibody (7A6C10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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