WDHD1 Antibody

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091]

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human testis shows nuclear positivity in cells in seminiferous ducts.

Western Blot: WDHD1 Antibody [NBP1-89091]

Western Blot: WDHD1 Antibody [NBP1-89091] - Analysis in human cell line RH-30.

Western Blot: WDHD1 Antibody [NBP1-89091]

Western Blot: WDHD1 Antibody [NBP1-89091] - The interaction of DDX11 with WDHD1 does not depend on FeS cluster binding. Reciprocal co-immunoprecipitations of Flag-tagged WDHD1 and untagged DDX11, and Flag-tagged DDX11 and myc-tagged WDHD1, respectively, extracted from 293T cells. Image collected and cropped by CiteAb from the following publication (https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.201900547), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091]

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human liver shows no positivity in hepatocytes.

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091]

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human lymph node shows strong nuclear positivity in germinal center cells.

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091]

Immunohistochemistry-Paraffin: WDHD1 Antibody [NBP1-89091] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Western Blot: WDHD1 Antibody [NBP1-89091] -

Western Blot: WDHD1 Antibody [NBP1-89091] - DDX11 interacts with Pol delta independently of its FeS cluster.(A) Gene Ontology term enrichment analysis of interaction partners obtained upon pull-down of YFP-DDX11 from HeLa Flp-In T-REx cells. (B) Flag pull-down of over-expressed Flag-tagged DDX11 from 293T cells & co-immunoprecipitated endogenous proteins. (C) Reciprocal co-immunoprecipitations of Flag-tagged POLD1 & untagged DDX11, & Flag-tagged DDX11 & untagged POLD1, respectively, extracted from 293T cells. (D) Co-immunoprecipitations of Flag-tagged DDX11 variants & untagged POLD1 from 293T cells. See also Fig S3, Tables S1, & S2. WB, Western blot. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32071282), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WDHD1 Antibody [NBP1-89091] -

Western Blot: WDHD1 Antibody [NBP1-89091] - CST is required for AND-1 & pol alpha chromatin association.(A) Representative images of pre-extracted HeLa cells used to measure chromatin-associated AND-1. DAPI: blue, AND-1: red. Scale bar = 12.5 μm. (B) Dot plots of mean AND-1 intensity per nuclei in AFU for each cell line, as indicated. Black line & numbers below the graph indicate the mean AFU. Error bars indicate the ±SEM of three independent biological experiments. (C) Western blot analysis showing chromatin fractions from HCT116 cells. Ponceau S & histone H3 were used as loading controls. AND-1 & pol alpha levels were normalized to H3 levels & then normalized to the shNT control. (D) Co-IP was performed with Flag or HA antibody in cell lysates from HEK 293T cells, as indicated. 5% input was loaded as a control. For +HU samples in (B) & (D), HU was added 2 h before collection. Data are representative of three independent biological experiments. n indicates the number of total nuclei scored. P-values were calculated by an unpaired, two-tailed Mann–Whitney test in (B) & t test in (C) (****P ≤ 0.0001, **P ≤ 0.01). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30979824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: WDHD1 Antibody [NBP1-89091] -

Immunocytochemistry/ Immunofluorescence: WDHD1 Antibody [NBP1-89091] - CST is required for AND-1 & pol alpha chromatin association.(A) Representative images of pre-extracted HeLa cells used to measure chromatin-associated AND-1. DAPI: blue, AND-1: red. Scale bar = 12.5 μm. (B) Dot plots of mean AND-1 intensity per nuclei in AFU for each cell line, as indicated. Black line & numbers below the graph indicate the mean AFU. Error bars indicate the ±SEM of three independent biological experiments. (C) Western blot analysis showing chromatin fractions from HCT116 cells. Ponceau S & histone H3 were used as loading controls. AND-1 & pol alpha levels were normalized to H3 levels & then normalized to the shNT control. (D) Co-IP was performed with Flag or HA antibody in cell lysates from HEK 293T cells, as indicated. 5% input was loaded as a control. For +HU samples in (B) & (D), HU was added 2 h before collection. Data are representative of three independent biological experiments. n indicates the number of total nuclei scored. P-values were calculated by an unpaired, two-tailed Mann–Whitney test in (B) & t test in (C) (****P ≤ 0.0001, **P ≤ 0.01). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30979824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: WDHD1 Antibody [NBP1-89091] -

Western Blot: WDHD1 Antibody [NBP1-89091] - CST is required for AND-1 & pol alpha chromatin association.(A) Representative images of pre-extracted HeLa cells used to measure chromatin-associated AND-1. DAPI: blue, AND-1: red. Scale bar = 12.5 μm. (B) Dot plots of mean AND-1 intensity per nuclei in AFU for each cell line, as indicated. Black line & numbers below the graph indicate the mean AFU. Error bars indicate the ±SEM of three independent biological experiments. (C) Western blot analysis showing chromatin fractions from HCT116 cells. Ponceau S & histone H3 were used as loading controls. AND-1 & pol alpha levels were normalized to H3 levels & then normalized to the shNT control. (D) Co-IP was performed with Flag or HA antibody in cell lysates from HEK 293T cells, as indicated. 5% input was loaded as a control. For +HU samples in (B) & (D), HU was added 2 h before collection. Data are representative of three independent biological experiments. n indicates the number of total nuclei scored. P-values were calculated by an unpaired, two-tailed Mann–Whitney test in (B) & t test in (C) (****P ≤ 0.0001, **P ≤ 0.01). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30979824), licensed under a CC-BY license. Not internally tested by Novus Biologicals.