Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all WDR5 Antibodies »

Product Summary for WDR5 Antibody

Immunogen

maps to a region between residue 1 and 50 of human WD repeat domain 5 using the numbering given in entry NP_060058.1

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WDR5 Antibody

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063]

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063]

Immunohistochemistry-Paraffin: WDR5 Antibody [NBP1-30063] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 WDR5 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: WDR5 Antibody [NBP1-30063]

Immunoprecipitation: WDR5 Antibody [NBP1-30063]

Immunoprecipitation: WDR5 Antibody [NBP1-30063] - Detection of human WDR5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody A302-429A (lot A302-429A-2) used for IP at 6 ug per reaction. WDR5 was also immunoprecipitated by rabbit anti-WDR5 antibody A302-430A. For blotting immunoprecipitated WDR5, A302-430A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for WDR5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 - 10 ug/mg lysate
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. Western Blot Not recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WDR5

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longer transcript. The two variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

WD Repeat-containing Protein 5

Alternate Names

BIG3, SWD3

Entrez Gene IDs

11091 (Human)

Gene Symbol

WDR5

UniProt

P61964

Additional WDR5 Products

Product Documents for WDR5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WDR5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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