WHIP Antibody

Western Blot: WHIP Antibody [NBP1-77987]

Western Blot: WHIP Antibody [NBP1-77987] - Antibody with and without pre-incubation with blocking peptide. Testing was performed on antiserum prior to affinity purification. Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1. ~30ug of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kDa is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(R) 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min.

Western Blot: WHIP Antibody [NBP1-77987]

Western Blot: WHIP Antibody [NBP1-77987] - HEK293 whole cell lysate. ~30ug of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye(R) 800 labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min.