WIPF1/WIP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-30642

Novus Biologicals, part of Bio-Techne
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NBP3-30642
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WIPF1/WIP Antibodies »

Product Specifications

Immunogen

Between 150 and 200

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WIPF1/WIP Antibody

WIPF1/WIP Antibody

Immunoprecipitation:WIPF1/WIP AntibodyNBP3-30642] -

Immunoprecipitation:WIPF1/WIP AntibodyNBP3-30642] -Detection of human WIPF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-WIPF1 antibody NBP3-30642 (lot NBP3-30642-1) used for IP at 6 µg per reaction. WIPF1 was also immunoprecipitated by rabbit anti-WIPF1 antibody BL21323. For blotting immunoprecipitated WIPF1, NBP3-30642 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for WIPF1/WIP Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate pH 7 -8.

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: WIPF1/WIP

WIPF1 is involved in the organization of the actin cytoskeleton. The protein binds to a region of Wiskott-Aldrich syndrome protein that is frequently mutated in Wiskott-Aldrich syndrome, an X-linked recessive disorder. Impairment of the interaction between these WIPF1 and Wiskott-Aldrich syndrome protein may contribute to the disease. Two transcript variants encoding the same protein have been identified for this gene. WIPF1 binds to Wiskott-Aldrich syndrome protein, profilin and actin. It is involved with NCK1 and GRB2 in the recruitment and activation of N-WASP and may be involved in regulating the subcellular localization of N-WASP, resulting in the disassembly of stress fibers in filopodia formation.

Alternate Names

WAS/WASL interacting protein family, member 1, WAS/WASL-interacting protein family member 1, WASP-interacting protein, WASPIP, WIPMGC111041, WiskProtein PRPL-2, WiskPRPL-2

Gene Symbol

WIPF1

Additional WIPF1/WIP Products

Product Documents for WIPF1/WIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WIPF1/WIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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