WISP-1/CCN4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86570

Novus Biologicals, part of Bio-Techne
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NBP1-86570
NBP1-86570-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WISP-1/CCN4 Antibodies »

Product Summary for WISP-1/CCN4 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QVVGVGCVLDGVRYNNGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WISP-1/CCN4 Antibody

Immunocytochemistry/ Immunofluorescence: WISP-1/CCN4 Antibody [NBP1-86570]

Immunocytochemistry/ Immunofluorescence: WISP-1/CCN4 Antibody [NBP1-86570]

Immunocytochemistry/Immunofluorescence: WISP-1/CCN4 Antibody [NBP1-86570] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Applications for WISP-1/CCN4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WISP-1/CCN4

WISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Long Name

Wnt-1 Induced Secreted Protein 1

Alternate Names

CCN4, WISP1

Gene Symbol

CCN4

Additional WISP-1/CCN4 Products

Product Documents for WISP-1/CCN4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WISP-1/CCN4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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