Wnt-1 Antibody (10C8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51575

Novus Biologicals, part of Bio-Techne
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NBP1-51575
NBP1-51575-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10C8

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Wnt-1 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of Wnt-1 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-1 Antibody (10C8) - BSA Free

Western Blot: Wnt-1 Antibody (10C8)BSA Free [NBP1-51575]

Western Blot: Wnt-1 Antibody (10C8)BSA Free [NBP1-51575]

Western Blot: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: Wnt-1 Antibody (10C8) - BSA Free [NBP1-51575]

Immunocytochemistry/ Immunofluorescence: Wnt-1 Antibody (10C8) - BSA Free [NBP1-51575]

Immunocytochemistry/Immunofluorescence: Wnt-1 Antibody (10C8) [NBP1-51575] - Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) - BSA Free [NBP1-51575]

Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) - BSA Free [NBP1-51575]

Immunohistochemistry-Paraffin: Wnt-1 Antibody (10C8) [NBP1-51575] - Analysis of human LAdrenal tissues using WNT1 mouse mAb.
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Applications for Wnt-1 Antibody (10C8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-1

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt1 (Wingless-type MMTV integration site family member 1) is a member of the WNT gene family. It is highly conserved in evolution and the protein encoded by this gene is known to be 98% identical to mouse Wnt1 protein at the amino acid level. Studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. Wnt1 is secreted as an extracellular matrix protein.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Alternate Names

Int-1, Wnt1

Gene Symbol

WNT1

Additional Wnt-1 Products

Product Documents for Wnt-1 Antibody (10C8) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-1 Antibody (10C8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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