Wnt-3a Antibody (1E6G4) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52588
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1E6G4
Format
BSA Free
Concentration
1 mg/ml
Product Summary for Wnt-3a Antibody (1E6G4) - BSA Free
Immunogen
Purified recombinant fragment of human Wnt-3a (AA: 170-352) expressed in E. Coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
39.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Wnt-3a Antibody (1E6G4) - BSA Free
Western Blot: Wnt-3a Antibody (1E6G4)BSA Free [NBP2-52588]
Western Blot: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis using WNT3A mouse mAb against A549 (1), HepG2 (2), and A431 (3) cell lysate.Immunocytochemistry/ Immunofluorescence: Wnt-3a Antibody (1E6G4) - BSA Free [NBP2-52588]
Immunocytochemistry/Immunofluorescence: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of HeLa cells using WNT3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) - BSA Free [NBP2-52588]
Immunohistochemistry-Paraffin: Wnt-3a Antibody (1E6G4) [NBP2-52588] - Analysis of endometrial cancer tissues, mouse mab with DAB staining.Applications for Wnt-3a Antibody (1E6G4) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1:200-1:400
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Wnt-3a
Long Name
Wingless-type MMTV Integration Site Family, Member 3a
Alternate Names
Wnt3a
Gene Symbol
WNT3A
Additional Wnt-3a Products
Product Documents for Wnt-3a Antibody (1E6G4) - BSA Free
Product Specific Notices for Wnt-3a Antibody (1E6G4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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