Wnt-3a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-74183

Novus Biologicals, part of Bio-Techne
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NBP1-74183
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Wnt-3a Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to the C terminal of Wnt3a. Immunizing peptide sequence IGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEA. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Wnt-3a Antibody

Western Blot: Wnt-3a Antibody [NBP1-74183]

Western Blot: Wnt-3a Antibody [NBP1-74183]

Western Blot: Wnt-3a Antibody [NBP1-74183] - Sample Tissue: Mouse Heart, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 1ug/ml, Peptide Concentration: 5ug/ml, Lysate Quantity: 25ug/lane, Gel Concentration: 0.12%
Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183]

Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183]

Immunohistochemistry-Paraffin: Wnt-3a Antibody [NBP1-74183] - Human placenta tissue at an antibody concentration of Dilution: 1:500.
Western Blot: Wnt-3a Antibody [NBP1-74183]

Western Blot: Wnt-3a Antibody [NBP1-74183]

Western Blot: Wnt-3a Antibody [NBP1-74183] - Titration: 1 ug/ml Positive Control: Mouse Heart lysate.
View 1 more images

Applications for Wnt-3a Antibody

Application
Recommended Usage

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-3a

Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube.

Long Name

Wingless-type MMTV Integration Site Family, Member 3a

Alternate Names

Wnt3a

Gene Symbol

WNT3A

UniProt

P27467

Additional Wnt-3a Products

Product Documents for Wnt-3a Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Wnt-3a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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