Wnt-5a Antibody (3A4)

Novus Biologicals, part of Bio-Techne | Catalog # H00007474-M04

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3A4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Wnt-5a Antibodies »

Product Specifications

Immunogen

WNT5A (AAH64694, 201 a.a. ~ 300 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ERERIHAKGSYESARILMNLHNNEAGRRTVYNLADVACKCHGVSGSCSLKTCWLQLADFRKVGDALKEKYDSAAAMRLNSRGKLVQVNSRFNSPTTQDLV

Specificity

Reacts with wingless-type MMTV integration site family, member 5A.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Wnt-5a Antibody (3A4)

Immunohistochemistry-Paraffin: Wnt-5a Antibody (3A4) [H00007474-M04]

Immunohistochemistry-Paraffin: Wnt-5a Antibody (3A4) [H00007474-M04]

Immunohistochemistry-Paraffin: Wnt-5a Antibody (3A4) [H00007474-M04] - Analysis of monoclonal antibody to WNT5A on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml
ELISA: Wnt-5a Antibody (3A4) [H00007474-M04]

ELISA: Wnt-5a Antibody (3A4) [H00007474-M04]

ELISA: Wnt-5a Antibody (3A4) [H00007474-M04] - Detection limit for recombinant GST tagged WNT5A is 3 ng/ml as a capture antibody.

Applications for Wnt-5a Antibody (3A4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Wnt-5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Entrez Gene IDs

7474 (Human)

Gene Symbol

WNT5A

UniProt

AAH64694 (Human)

Additional Wnt-5a Products

Product Documents for Wnt-5a Antibody (3A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Wnt-5a Antibody (3A4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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