Wnt-5a Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-60032

Novus Biologicals, part of Bio-Techne
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NBP1-60032
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all Wnt-5a Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to WNT5A(wingless-type MMTV integration site family, member 5A) The peptide sequence was selected from the middle region of WNT5A. Peptide sequence GGCGDNIDYGYRFAKEFVDARERERIHAKGSYESARILMNLHNNEAGRRT. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29563288).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Wnt-5a Antibody

Western Blot: Wnt-5a Antibody [NBP1-60032]

Western Blot: Wnt-5a Antibody [NBP1-60032]

Western Blot: Wnt-5a Antibody [NBP1-60032] - Hela Cell Lysate 1.0 ug/ml, gel concentration 12%
Immunohistochemistry: Wnt-5a Antibody [NBP1-60032]

Immunohistochemistry: Wnt-5a Antibody [NBP1-60032]

Immunohistochemistry: Wnt-5a Antibody [NBP1-60032] - Human Adult heart Observed Staining: Cytoplasmic,Membrane Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.
Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP1-60032]

Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP1-60032]

Immunohistochemistry-Paraffin: Wnt-5a Antibody [NBP1-60032] - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
View 2 more images

Applications for Wnt-5a Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-5a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT5A is a member of the WNT protein family. It shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

Wingless-type MMTV Integration Site Family, Member 5a

Alternate Names

Wnt5a

Gene Symbol

WNT5A

UniProt

P41221

Additional Wnt-5a Products

Product Documents for Wnt-5a Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Wnt-5a Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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