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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Predicted:

Rabbit (93%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid peptide near the center of human WNT5B. The immunogen is located within amino acids 130 - 180 of WNT5. Amino Acid Squence: CSRTARPKDLPRDWL

Specificity

WNT5 antibody will detect both WNT5A and WNT5B. At least two isoforms of WNT5B are known to exist; this antibody will detect both isoforms.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

465 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Wnt-5b Antibody - BSA Free

Western Blot: Wnt-5b AntibodyBSA Free [NBP2-41306]

Western Blot: Wnt-5b AntibodyBSA Free [NBP2-41306]

Western Blot: Wnt-5b Antibody [NBP2-41306] - Western blot analysis of WNT-5b in Jurkat cell lysate with WNT-5b antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Wnt-5b Antibody - BSA Free

Immunohistochemistry: Wnt-5b Antibody - BSA Free [NBP2-41306] -

Immunohistochemistry: Wnt-5b Antibody - BSA Free [NBP2-41306] - Immunohistochemistry of Wnt-5b in rat colon tissue with Wnt-5b antibody at 5 ug/mL.

Applications for Wnt-5b Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-5b

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteinshave been implicated in oncogenesis and in several developmental processes, including regulation of cell fate andpatterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94%and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicingof this gene generates 2 transcript variants. (provided by RefSeq)

Long Name

Wingless-type MMTV Integration Site Family, Member 5b

Alternate Names

Wnt5b

Gene Symbol

WNT5B

Additional Wnt-5b Products

Product Documents for Wnt-5b Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-5b Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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