Wnt3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-40793
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence CGRGHNTRTEKRKEK corresponding to internal region according to NP_110380.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Wnt3 Antibody
Western Blot: Wnt3 Antibody [NB100-40793]
Western Blot: Wnt3 Antibody [NB100-40793] - HEK293 cell lysate (35 ug protein in RIPA buffer). Primary antibody at 1 ug/ml, 1 hr incubation. Detected by chemiluminescence.Immunohistochemistry-Paraffin: Wnt3 Antibody [NB100-40793]
Immunohistochemistry-Paraffin: Wnt3 Antibody [NB100-40793] - Paraffin embedded human breast tissue. Antibody at 2 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Wnt3 Antibody
Application
Recommended Usage
ELISA
Detection limit 1:16000
Immunohistochemistry
2-4 ug/ul
Immunohistochemistry-Paraffin
2-4 ug/ml
Western Blot
1 - 3 ug/ml
Application Notes
Western blot: Approx 40 kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6 kDa according to NP_110380.1).
IHC: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells.
IHC: In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Wnt-3
Long Name
Wingless-type MMTV Integration Site Family, Member 3
Alternate Names
INT4, TETAMS, WNT3
Gene Symbol
WNT3
UniProt
Additional Wnt-3 Products
Product Documents for Wnt3 Antibody
Product Specific Notices for Wnt3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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